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. Author manuscript; available in PMC: 2019 Oct 1.
Published in final edited form as: Biochim Biophys Acta Mol Basis Dis. 2018 Aug 8;1864(10):3527–3536. doi: 10.1016/j.bbadis.2018.08.007

Figure 4.

Figure 4.

Exposure to suPAR increases abundance of proteins phosphorylated on tyrosine residues and causes ROS-dependent activation of Src. (a) Immunoblot analysis showing increased abundance of phosphotyrosine in podocytes treated with suPAR for 24 hr. (b) Immunoblot showing increased abundance of Src-pY418 relative to total Src in cells treated with suPAR. Exposing podocytes to medium containing 1 mM H2O2 for 30 min increased abundance of phosphortyrosine (c) and increased the abundance of Src-pY418 relative to total Src (d). (e) suPAR-evoked increases in SrcpY418 relative to total Src were blocked by concurrent exposure to TEMPOL.