Figure 1.

PIP 640 fails to affect cellular levels or distribution of TAMPs (occludin, tricellulin, and marvelD3) as well as the scaffolding protein ZO-1 in Caco-2 cell monolayers in vitro. A) Representative immunoblots of TAMPS and ZO-1 total protein levels showed no striking changes following a 60-min apical exposure of 1 mM PIP 640; conditions that increased MLC-pS¹⁹ relative to total MLC levels. B) Quantitative analysis of immunoblots as shown in A); data are means ± SEM of 3 independent experiments, n=6 for control and treated monolayers. C) Immunofluorescence microscopy images demonstrated no dramatic differences in localization of TAMPs and ZO-1 had not changed following a 60min apical exposure of 1 mM PIP 640 compared to untreated Caco-2 monolayers in vitro. Images are representative of 3 independent experiments, n=3. Scale bar, 10 μm.