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. Author manuscript; available in PMC: 2019 Oct 28.
Published in final edited form as: J Control Release. 2018 Sep 6;288:111–125. doi: 10.1016/j.jconrel.2018.09.003

Figure 4.

Figure 4.

PIP 640-mediated shift in distribution of claudin-2 from nuclear to membrane fractions. Confluent Caco-2 cell monolayers were gently disrupted 60 min after an apical application of 1 mM PIP 640 in vitro. A) Representative immunoblots for claudin-2 and occludin content in isolated nuclear and membrane fractions. B) Quantification of immunoblot band intensity for the relative levels of claudin-2 and occludin following this PIP 640 treatment. Data are means ± SEM of 3 independent experiments, n=3 for the control and treated monolayers, (*p value< 0.05).

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