Figure 5.

Time-course assessment claudin-2 and MLC-pS¹⁹ levels with relation to changes in transepithelial electrical resistance (TEER) resistance in Caco-2 cell monolayers following apical treatment with 1 mM PIP 640 in vitro. A) TEER changes induced by 1 mM of PIP 640 over 60-min exposure following by recovery initiated by the removal of apical PIP 640 (arrow) and replacement with fresh media. B) Representative immunoblots showing MLC-pS¹⁹ levels relative to total MLC and claudin-2 levels over time following apical application of 1 mM PIP 640. C) Quantitative analysis of immunoblots obtained for MLC-pS¹⁹ and claudin-2 induced by 1 mM PIP 640. Data points represent means ± SEM of 3 independent experiments with n=6 for TEER data, n= 3 for both MLC-pS¹⁹ and claudin-2 immunoblotting data at all time points, immunoblot data obtained for MLC-pS¹⁹ for 40 min exposure n=1 (*p value< 0.05, **p value< 0.01 and ***p value<0.001).