Figure 7.

Actions of PIP 644 are distinct from PIP 640 on Caco-2 cell monolayers in vitro. A) Representative immunoblots showing cellular levels of MLC-pS¹⁹ and claudin-2 at 10 and 60 min following apical application of 1 mM of PIP 640 or PIP 644. B) Quantitative analysis of immunoblots obtained for MLC-pS¹⁹ and claudin-2 induced by PIP 640 or PIP 644 represented in A). C) Representative immunoblots showing cellular levels of MLC-pS¹⁹ and occludin at 60 min following apical application of 1 mM of PIP 640 or PIP 644. D) Quantitative analysis of immunoblots obtained for MLC-pS¹⁹ and occludin induced by PIP 640 or PIP 644 represented in C). Data represent means ± SEM of 3 independent experiments with n=3 for both MLC-pS¹⁹ and claudin-2 immunoblotting data at all time points (**p value< 0.01). E) Immunofluorescent staining of claudin-2 and occludin in Caco-2 cell monolayers treated with PIP 640 or PIP 644 for 60 min. Images are representative of 3 independent experiments, n=3. Scale bar, 10 μm