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. 2018 Nov 6;9:4634. doi: 10.1038/s41467-018-07009-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Disruption of DYNAMO1 functions and whole-mount negative-staining electron microscopy analysis of DYNAMO1-Dnm1 dynamics. a Profiles of mitochondrial division based on morphological changes during division. Mitochondria are labeled as in Fig. 2a. n = 3, at least 50 cells were counted in each experiment. b Localization of expressed DYNAMO1-HA and DYNAMO1 H116D-HA (Alexa 488-conjugated anti-HA antibody), Dnm1, and mitochondria in metaphase cells. c Length of mitochondria in cells expressing DYNAMO1-HA or DYNAMO1 H116D-HA. n = 50 in each experiment. Orange dots indicate hyper-constricted mitochondria as determined by quantification of their length that was elongated by more than 1.5 μm, which were positive for DYNAMO1 and Dnm1. d Ratio of hyper-constricted mitochondria during the constriction phase. e Profiles of peroxisomal division based on morphological changes during division. n = 3, at least 50 cells were counted in each experiment. f Localization of DYNAMO1-HA, DYNAMO1 H116D-HA, Dnm1, and peroxisomes in anaphase cells. g Lengths of peroxisomes in cells expressing DYNAMO1-HA or DYNAMO1 H116D-HA. n = 50 in each experiment. Orange dots indicate hyper-constricted peroxisomes as determined in c by quantification of their length that was elongated by more than 1 μm, which were positive for DYNAMO1 and Dnm1. h Ratio of hyper-constricted peroxisomes during the constriction phase. Scale bars: 1 μm. Data are means ± s.d. P; p-value (Mann–Whitney test)