Table 2.

Notation of nucleosome constructs

Notation	Sample description
IβIα-wt	DNA labeled at +41 (A488) and −53 (A594) and wt octamers
IβIα-mut	DNA labeled at +41 (A488) and −53 (A594) and octamers containing H2A-R81A mutated histones
EβDyα	DNA labeled at 5′ end β (Eβ) with A488 and at position −15 (close to the dyad) on the other strand (Dyα) with A594, plus wt octamers
EαDyβ	Analogous to nucleosome EβDyα with DNA label positions on the complementary strands (A488 at 5′ end α, A594 at + 15)
H2B-Dyα	DNA labeled at -15 (near the dyad) with Cy5 and octamers containing H2B-A488