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. 2018 Nov 6;8:16440. doi: 10.1038/s41598-018-34814-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fold changes of differentiated metabolites after acute TPHP expousre: (A) asparagine, (B) leucine, (C) lysine, (D) phenylalanine, (E) proline, (F) serine, (G) threonine, (H) valine, (I) glucoside, (J) inosine and (K) phospholipids. For A-J, fold changes were calculated by dividing the peak areas of the exposure groups with the mean peak area of the control group for the same day from the GC-MS chromatograms. For phospholipids, control group, “Control” refers to control-day 1 and day 2 samples, whereas “Exposure” refers to the pool of the rest of the samples, and fold changes were calculated by dividing peak area in “Exposure” with mean peak area in “Control”. Data are shown as mean ± standard deviation. Significantly different levels compared with related control are labeled with an asterisk (at P < 0.05) or two asterisks (at P < 0.01) based on a two-tailed student t-test.