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. 2018 Nov 6;8:16437. doi: 10.1038/s41598-018-34773-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Spawning induction and in vitro fertilization in Aiptasia. (A) Culture and induction regime provides gametes throughout the week. Percentage of spawning events on each weekday (total from 24 weeks). (B) Female animal with egg patch (arrowhead). Scale = 5 mm. (C) Gelatin coating increases fertilization success. n = 3; p < 0.01; error bars, s.d. (D) Fertilization success rapidly decreases over time after spawning (n = 5). Error bars, s.d. (E) Zygotes are transferred into a microinjection dish filled with FASW, where they sink into holes in the mesh. Scale = 200 μm. (D) Co-injection of dextran-AlexaFluor 594 allows identification of successfully injected zygotes. Injected zygotes are brightly fluorescent whereas uninjected zygotes appear dark (arrowhead). Scale = 200 μm.