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. 2018 Nov 6;8:16437. doi: 10.1038/s41598-018-34773-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Microinjection of DNA into Aiptasia zygotes. (A) Schematic of injected plasmids: the promoter of an Aiptasia actin gene drives expression of the NLS-eGFP-V2A-mCherry-CaaX reporter with a SV40 termination sequence. Meganuclease I-SceI recognition sites are indicated. (B) The promoter of actin gene XM_021049442.1 drives reporter expression in the majority of larvae coinjected with plasmid and the meganuclease I-SceI. For each promoter tested, the expression level (FPKM) (larvae transcriptomes¹⁹), the number of larvae injected and analyzed, and the percentage of larvae in which GFP signal could be detected are given. (C) Coinjection of I-SceI and the _prom XM_021049442.1: NLS-eGFP-V2A-mCherry-CAAX:SV40 plasmid causes strong, mosaic expression of the transgene, labeling nuclei (green) and membranes (magenta). 10 hpf, scale = 25 μm.