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. 2018 Nov 6;8:16427. doi: 10.1038/s41598-018-34500-w

Figure 3.

Figure 3

Effect of Trichoderma inoculation on expression of DR5:uidA at 22 °C. Seven day-old DR5:uidA Arabidopsis seedlings were inoculated with T. atroviride (Ta) and T. virens (Tv) spores on MS and PDA media for 5 days. The following conditions were analyzed: direct interaction (At-MS), split interaction without contact when the fungus was grown on MS medium (At/MS), split interaction without contact when the fungus was grown on PDA medium (At/PDA), and as control plantlets grown on MS without any inoculation. GUS histochemical analysis was performed for each treatment. Photographs are representative of individuals from at least 15 stained GUS seedlings. The scale bar corresponds to 100 µm. Images of main and lateral roots were acquired in a Zeiss Axio Imager M2 microscope with DIC contrast at 10x magnification, and processed in ZEN software.