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. 2018 Nov 6;8:16427. doi: 10.1038/s41598-018-34500-w

Figure 4.

Figure 4

Effect of Trichoderma on root-hair formation in rhd6 mutant. (A) 3 day-old rhd6 mutant seedlings were inoculated with T. atroviride (Ta) and T. virens (Tv) for 5 days under the follow conditions: direct interaction (At-MS), split interaction without contact when the fungus was grown on MS medium (At/MS), split interaction without contact when the fungus was grown on PDA medium (At/PDA), and as control plantlets grown on MS without inoculation. Images were taken under a stereoscopic microscope (Motic smz-143 series) and are representative individuals from 10 seedlings. The scale bar corresponds to 500 µm. (B) Root hairs were counted in a region of 500 to 1000 μm from the main root tip. Density data were expressed in 500 μm area represented as mean ± SE (n = 10). Letters refer to bootstrap pairwise two-sample robust tests, treatments without shared letters are significantly different.