Table 2.
Identified BRB phytochemicals with P > 0.05 or fold change < 2
| Ret. time (min) |
Tentative ID | Observed [M-H]- | Major fragments | Chemical formula | Δ ppm | Fold changea |
|---|---|---|---|---|---|---|
| Flavonols | ||||||
| 7.36 | Quercetin petosyl-rutinosidec | 741.1864 | 301.0322 | C32H38O20 | −2.7 | - |
| 7.97 | Quercetin hexuronidec | 477.0668 | 301.0354 | C21H18O13 | −1.3 | −1.09 |
| 8.10 | Quercetin rutinosideb | 609.1447 | 301.0345 | C27H30O16 | −2.3 | −1.08 |
| 8.22 | Quercetin glucosideb | 463.0867 | 301.0351 | C21H20O12 | −3.2 | −1.13 |
| 8.63 | Myricetin glucosidec | 479.0813 | 317.0299 | C21H20O13 | −3.8 | −1.12 |
| 9.60 | Quercetinb | 301.0345 | 151.0032 | C15H10O7 | −3.0 | 1.15 |
| Procyanidins | ||||||
| 4.60 | B-type procyanidinc | 577.134 | 289.0714 | C30H26O12 | −2.1 | - |
| 4.63 | Catechinb | 289.0714 | 245.0815 | C15H14O6 | −1.4 | - |
| 5.03 | B-type procyanidinc | 577.134 | 289.0714 | C30H26O12 | −2.1 | −1.23 |
| 5.28 | Epicatechinb | 289.0715 | 245.0817 | C15H14O6 | −1.0 | 1.17 |
| Phenolic acids | ||||||
| 1.40 | Gallic acidb | 169.0146 | 125.0243 | C7H6O5 | 2.4 | 1.92 |
| 3.06 | Salicylic acid hexosidec | 299.0773 | 137.0246 | C13H16O8 | 0.33 | - |
| 4.23 | Caffeic acid hexosidec | 341.087 | 179.0352 | C15H18O9 | −2.3 | −1.14 |
| 5.00 | Coumaric acid hexosidec | 325.0923 | 163.0399 | C15H18O8 | −1.8 | −1.24 |
| 5.11 | Caffeic acidb | 179.0361 | 135.0452 | C9H8O4 | 6.1 | 1.71 |
| 5.28 | Salicylic acidb | 137.0247 | C7H6O3 | 2.2 | 1.69 | |
aMean abundance in nectar vs BRB powder, fold change shown for compounds with P<0.05, Identified level Ib, IIc, IIId, according to the Metabolomics Standards Initiative