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. Author manuscript; available in PMC: 2019 Dec 1.
Published in final edited form as: Inflamm Res. 2018 Oct 17;67(11-12):1013–1019. doi: 10.1007/s00011-018-1196-x

Figure 4.

Figure 4.

PKA requires SIRT1 to decrease cytoplasmic HMGB1. REC were grown in normal glucose (5mM, NG), high glucose (25mM, HG), HG+forskolin (HG+Fors), HG+Ex527 (Sirt1 inhibitor), or HG+Ex527+Forskolin (HG+Fors+Ex527). Panel A shows that forskolin increased PKA activity. Panel B shows that Ex527 inhibited SIRT1, Panel C is cytoplasmic HMGB1, and Panel D is nuclear HMGB1 levels. *P<0.05 vs. NG, #P<0.05 vs. HG, $P<0.05 vs. HG+Fors. Data are mean ± SEM. N=4 for all groups.