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Schematic of dietary zinc manipulation and Clostridium difficile infection. C57BL/6 mice are given altered diets for 5-weeks and treated with cefoperazone in their drinking water for 5 days. Following 2-days of recovery, mice are infected with C. difficile via oral gavage. Fresh fecal samples are collected for microbiota analysis during diet manipulation and antibiotic treatment, and fecal samples are utilized to monitor bacterial burden and toxin titers following infection.
