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Hyp enrichment medium composition.
| Component | Concentration |
|---|---|
| NaCl | 100 g/L |
| MgCl2·6H2O | 40 g/L |
| CaCl2·2H2O | 10 g/L |
| KCl | 50 g/L |
| NH4Cl | 10 mM |
| KH2PO4 | 4 g/L |
| K2HPO4 | 12.7 g/L |
| MOPS buffer, pH 7.2 | 10 mM |
| Sodium formate | 40 mM |
| Sodium acetate | 1 mM |
| Hyp | 20 mM |
| Thiosulfate | 0.25 mM |
| Sodium sulfide | 0.25 mM |
| Sodium 2-bromoethanesulfonate* | 10 mM |
| ATCC trace mineral | 1% |
| ATCC vitamin solution | 1% |
| Resazurin | 0.0001% |
*added after passaging 2–3 times.
