Skip to main content
. 2018 Oct 19;9(82):35313–35326. doi: 10.18632/oncotarget.26238

Figure 4. Hematologic characterization of a crossbred Wt1+/R394W x Flt3+/ITD mouse model.

Figure 4

(A) Kaplan–Meier survival curve of the crossbred mice containing both mutations (ITD/Wt1+/R394W) compared to mice with either mutation alone (Wt1+/R394W, p < 0.0001 or ITD, p < 0.0001) and compared to wild type (wt) littermates. (B) Representative peripheral blood smears from wt, ITD, and ITD/Wt1+/R394W mice(40×; scale bars are 10 microns). (C) Representative bone marrow cytospins from wt, ITD, and ITD/Wt1+/R394Wmice. Black arrows point to normal erythroid precursors, and red arrows indicate erythroid precursors with dysplastic changes in the ITD/Wt1+/R394W mice (top row 40×, bottom row 100×; all scale bars are 10 microns). (D) Comparison of myeloid to erythroid (M:E) ratios in the bone marrow of each genotype. Mean values for each genotype: wt 2.89 ± 0.12 (n = 40), Wt1+/R934W 2.13 ± 0.29 (n = 4), ITD 32.46 ± 5.94 (n = 12), ITD/Wt1+/R394W 10.11 ± 1.67 (n = 32). Horizontal bars represent the mean values, error bars represent the SEM. (E) Distribution of hematologic phenotype by mouse genotype. Phenotype definitions are based on the Bethesda classification, and the predominant phenotype in each genotype is highlighted. MDS = myelodysplastic syndrome, MPN = myeloproliferative neoplasm, AML = acute myeloid leukemia, T-ALL = T-cell acute lymphoblastic leukemia.