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. 2018 Nov 5;150(11):1510–1522. doi: 10.1085/jgp.201812104

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© 2018 Kawai et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

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Figure 1. — MHC isoform expression analysis with SDS-PAGE. MHC isoform composition of fiber bundles dissected from skinned soleus muscle from Neb-expressing control muscle (top) and Neb-deficient muscle (bottom). The left lane is a standard (Std) that was made by mixing soleus and tibialis cranialis muscle lysate, revealing type I, IIB and IIA(X) MHC bands; note that this electrophoresis system is unable to separate IIA and IIX MHC isoforms. Lanes 1 and 5 are examples of bundles that contained both type I and IIA(X) MHC; these were not used in the present study. Lanes 2, 3, 4, and 6 contain solely type I MHC, and they are examples of fiber bundles that were entered in the present work.