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. Author manuscript; available in PMC: 2018 Nov 6.
Published in final edited form as: Science. 2018 Mar 16;359(6381):1259–1263. doi: 10.1126/science.aan6042

Fig. 3. GDV1 associates with heterochromatin throughout the genome and triggers HP1 removal at ap2-g.

Fig. 3

(A) HP1 over input ratio track from 3D7/GDV1-GFP-DDOFF schizonts (38-44 hpi, TP3) (grey). ChIP-seq subtraction tracks display relative enrichment of GDV1-GFP-DD in 3D7/GDV1-GFP-DDON schizonts two (30-36 hpi, TP1), six (34-40 hpi, TP2) and ten (38-44 hpi, TP3) hours after Shield-1 addition (blue), and relative depletion of HP1 in 3D7/GDV1-GFP-DDON parasites at TP3 (green). (B) Correlation between GDV1-DD-GFP enrichment in 3D7/GDV1-GFP-DDON (34-40 hpi, TP2) and HP1 enrichment in 3D7/GDV1-GFP-DDOFF schizonts at each coding region. r, Pearson correlation coefficient. (C) Fold change in HP1 enrichment upon GDV1-GFP-DD overexpression in relation to HP1 enrichment in 3D7/GDV1-GFP-DDOFF schizonts for each heterochromatic gene. (D) Zoom-in view of the enrichment/subtraction tracks at the ap2-g locus.