REAGENT or RESOURCE	SOURCE	IDENTIFIER
Bacterial Strains
Escherichia coli K12 BW25115	Keio collection (Baba et al., 2006)	N/A
Escherichia coli K12 BW25113ΔsbmA	Keio collection (Baba et al., 2006)	Strain number JW0368-1
Escherichia coli K12 BW25113ΔyjiL	Keio collection (Baba et al., 2006)	Strain number JW5785-1
Escherichia coli K12 BW25113ΔyjiL/ΔsbmA	(Krizsan et al., 2015)	N/A
Escherichia coli N281	(Wittmann et al., 1973)	N/A
Escherichia coli N282	(Wittmann et al., 1973)	N/A
Escherichia coli AB301	(Bouck and Adelberg, 1970)	N/A
Escherichia coli SQ110ΔtolC	(Florin et al., 2017)	N/A
Escherichia coli SQ110ΔtolC A2059C	(Florin et al., 2017)	N/A
Escherichia coli SQ110ΔtolC A2059G	(Florin et al., 2017)	N/A
Escherichia coli SQ110ΔtolC A2305C	(Florin et al., 2017)	N/A
Thermus thermophilus HB8	DSMZ	Catalog # DSM-579
BL21 Star	Thermofisher	Catalog # C601003
Biological Samples
Tursiops truncates muscle tissue	Mediterranean Marine Mammal Tissue Bank	N/A
tRNA from Escherichia coli MRE600	Roche	Catalog # 10109550001
Thermus thermophilus 70S ribosomes	This study	N/A
Chemicals, Peptides, and Recombinant Proteins
YfiA protein	This study	N/A
Tur1A	This study	N/A
Tur1B	This study	N/A
Tur1A(Cys33)-BY	This study	N/A
Bac7(Cys36)-BY	This study	N/A
Myticalin A5	(Leoni et al., 2017)	N/A
Tur1A(1-16)	This study	N/A
Tur1A(8-24)	This study	N/A
Tur1A(16-32)	This study	N/A
Kanamycin	Sigma	Catalog # 60615
Erythromycin	Sigma	Catalog # E6376
Spectinomycin	Sigma	Catalog # S4014
Thiostrepton	Sigma	Catalog # T8902
Edeine	N/A	N/A
Fmoc-Arg(Pbf)-NovaSyn® TGA	Novabiochem	Catalog # 856042
Trifluoroacetic acid (TFA)	Sigma	Catalog # 91700
3,6-dioxa-1,8-octanedithiol (DODT)	Sigma	Catalog # 465178
Thioanisole	Sigma	Catalog # 88470
Triisopropylsilane (TIPS)	Sigma	Catalog # 233781
N,N'-diisopropylcarbodiimide (DIC)	Fluca	N/A
N-methylimidazole	VWR	Catalog # AAA12575-22
Dimethylformamide (DMF)	VWR	Catalog # BDH1117-4LG
Piperidine	Acros Organics	Catalog # P/3520
BDP FL maleimide	Lumiprobe	Catalog # 21480
N-Methyl-2-Pyrrolidinone	VWR	Catalog # CA71007-814
1-hydroxybenzotriazole hydrate (hOBt)	Sigma	Catalog # 54802
Prpidium iodide	Sigma	Catalog # P4170
RNasin	Promega	Catalog # N2511
rNTPs	Sigma	Catalog # 27-2025-01
PEG-8000	Sigma	Catalog # 1546605
AMV reverse transcriptase	NEB	Catalog # M0277
Hemo Klen Taq	NEB	Catalog # M0332
ddNTPs	Sigma	Catalog # GE27-2045-01
2-methyl-2,4-pentanediol (MPD)	Sigma	Catalog # 000000008208191000
Critical Commercial Assays
E.Z.N.A.® Mollusc DNA Kit	Omega Bio-Tek	Catalog # D3373
Perfectprep® Gel Clean-up Kit	Eppendorf	N/A
RTS™ 100 Escherichia coli HY	Biotech Rabbit	Catalog # BR1400101
Luciferase Assay System	Promega	Catalog # E1500
PURExpress in vitro transcription/translation kit	NEB	Catalog # E6800S
QIAquick Nucleotide Removal Kit	Qiagen	Catalog # 28304
Deposited Data
The crystal structure of the Tur1A-70S complex	This study	PDB ID 6FKR (https://www.rcsb.org/structure/6fkr)
Oligonucleotides
Tur1A FW 5’-CTTGTGACCCTGGGA-3’	Eurofins Genomics	N/A
Tur1A RV 5’-ATAACTTCCTCCAGGCTTCA-3’	Eurofins Genomics	N/A
NV-1 5’GGTTATAATGAATTTTGCTTATTAAC-3’	Thermo-Fisher	N/A
Recombinant DNA
H-ns-PPP	This study	N/A
pGS21A	(Polikanov et al., 2014)	N/A
2XermCL_S10K	(Arenz et al., 2016)	N/A
Software and Algorithms
Genomics Workbench 5.1 (CLCbio)	QIAGEN bioinformatics	https://www.qiagenbioinformatics.com/products/clc-genomics-workbench/
FCS Express3 software	De Novo Software	https://www.denovosoftware.com/site/DownloadLanding.shtml
XDS	(Kabsch, 2010)	http://xds.mpimf-heidelberg.mpg.de/html_doc/downloading.html
Phenix	(Adams et al., 2010)	https://www.phenix-online.org/download
Coot	(Emsley and Cowtan, 2004)	https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/
Other
Hitachi 3130 Genetic Analyzer	Applied Biosystems	N/A
Biotage Initiator+ Alstra	Biotage	Catalog # 356017
Kinetex, C18, 2.6 μm, 100 Å, 50x4,6 mm	Phenomenex	Catalog # 00A-4462-E0 00
Esquire 4000	Bruker Daltonics	N/A
NanoDrop2000	Thermo Fisher Scientific	Catalog # ND-2000
Jupiter™, C18, 5 μm, 300 Å, 100x10 mm	Phenomenex	Catalog # 00G-4053-E0
MultiPep RSi	Intavis	N/A
Shim-pack VP-ODS column (120 Å, 150x4.6 mm,)	Shimadzu	N/A
Cytomics FC 500	Beckman-Coulter	N/A
Tecan Infinite M1000	Tecan	N/A
M-110L Microfluidizer Processor	Microfluidics	N/A
Typhoon FLA9500	GE Healthcare	Catalog # 29187191
Protino Ni-NTA agarose beads	Macherey-Nagel	Catalog # 745400
Superdex HiLoad S75 16/600	GE Healthcare	Catalog # 28989333