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. 2018 Nov 5;217(11):3873–3885. doi: 10.1083/jcb.201804137

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© 2018 Condon et al.

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Figure 1. — LLSM of macrophage ruffling. RAW 264.7 macrophages stably expressing GFP-LifeAct and incubated in medium containing LPS were imaged using LLSM. (A) Single frame from LLSM recording (Video 1) visualized in 3D by tilting the field of view (FOV) to visualize the top and side of the cell. GFP-LifeAct fluorescence was visualized using the Orange-Hot Look Up Table (LUT) from the Amira data visualization software. (B) A second recording is depicted as an inverted grayscale MIP (Video 2) and then temporal color coding of 75 frames (1’45”) is illustrated in the right-hand panel. Bars, 10 µm.