Figure 1. .

Heterozygosity for Xrp1 rescues caz mutant pupal lethality. (A) Screening strategy to identify chromosomal deficiencies that rescue caz² pupal lethality. (B) Frequency of adult male offspring from the indicated cross that is heterozygous for a genomic caz transgene or the indicated deficiencies. n > 128 per genotype. ***, P < 0.0001; χ² test. (C) Genomic region uncovered by Df(3R)ED2. Pink indicates genes in the plus orientation; white indicates genes in the minus orientation. The different smaller deficiencies within this region which were tested for rescue of caz² pupal lethality are shown. Check marks indicate deficiencies that rescue; X marks indicate deficiencies that do not rescue. (D–F) Xrp1 genomic locus showing the insertion sites of the transposable elements used to generate Xrp1 mutant alleles. In the Df(3R)Xrp1^Plus allele (D), Xrp1, Mpc1, and the 5′ end of CG42613 are deleted. In the Xrp1^Ex-long allele (E), the 5′ half of Xrp1 is deleted, predicted to abolish expression of the Xrp1^Long isoform. The Xrp1^Short isoform, encoded by Xrp1-RD, may still be expressed. In the Xrp1^KO allele (F), the Xrp1 coding region is precisely deleted. (G and H) Xrp1 transcript levels in Xrp1 mutant lines relative to WT controls (100%) determined by qPCR using primers either selectively detecting Xrp1^Long transcripts (G) or detecting all Xrp1 transcripts (H). n = 10. ***, P < 0.0001; one-way ANOVA. Mean ± SEM. (I and J) Frequency of adult male offspring from the indicated crosses that is heterozygous for the indicated Xrp1 allele. n > 87 per genotype. *, P < 0.05; ***, P < 0.0001; χ² test.