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. 2018 Nov 5;217(11):3947–3964. doi: 10.1083/jcb.201802151

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© 2018 Mallik et al.

This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).

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Figure 4. — Xrp1 is a nuclear protein that binds chromatin. (A) Immunostaining of Drosophila S2R⁺ cells expressing N-terminal HA-tagged Xrp1^Short for lamin (labels the nuclear membrane), and the HA tag revealed Xrp1 localization to the nucleus (top). Immunostaining for Caz and HA::Xrp1 showed colocalization (bottom). (B) HA-tagged Xrp1 was selectively expressed in larval motor neurons (OK371-GAL4). Immunostaining for lamin and HA::Xrp1 revealed nuclear localization. Control animals are driver only. (C) HA-tagged Xrp1 was coexpressed with membrane-bound GFP in larval motor neurons. Immunolabeling for Caz and HA::Xrp1 showed colocalization of Caz and Xrp1. Control animals do not express HA::Xrp1. (D and E) Immunostaining of Xrp1 (D) or Caz (E) on polytene chromosomes. Chromatin was counterstained with DAPI. Xrp1 prominently localizes to euchromatic bands (arrows indicate examples), to puffs (arrowheads), and to centromeric β-heterochromatin (asterisks). Caz prominently localizes to puffs. Overall, Caz and Xrp1 display a distinct binding pattern, although there is some overlap, e.g., the puff region indicated by an arrowheads in D and E. Bars: 10 µm (A); 20 µm (B–E).