Figure 5. .

Identification of Xrp1- and Caz-interacting proteins. (A) Approach used to identify Xrp1-interacting proteins. N-terminal Flag-tagged Xrp1 was expressed in Drosophila S2 cells. Coimmunoprecipitation followed by quantitative MS was used to identify Xrp1-interacting proteins, defined as proteins significantly enriched in Flag::Xrp1-expressing cells as compared with control cells. (B) Venn diagrams illustrating the substantial overlap between Xrp1^Long-, Xrp1^Short-, and Caz-interacting proteins. (C) The majority (55.4%) of Xrp1-interacting proteins are involved in gene expression regulation or DNA/RNA metabolism, including regulation of transcription, chromatin organization, DNA metabolism, DNA repair, RNA metabolism, and DNA- or RNA-binding proteins.