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. 2018 Nov 5;217(11):3863–3872. doi: 10.1083/jcb.201801208

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© 2018 Arasaki et al.

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Figure 2. — Legionella effector DrrA interacts with the exocyst in a component-specific manner. (A) HEK293-FcγRII cells were cotransfected with plasmids encoding 3x-FLAG or 3x-FLAG-exocyst components and GFP-Stx2, 3, or 4. 24 h after transfection, cell lysates were prepared and immunoprecipitated (IP). (B) HEK293-FcγRII cells were cotransfected with plasmids encoding 3x-FLAG, 3x-FLAG-Exo70 or -Sec15, and GFP-DrrA_61–647 or -DrrA_451–647. 24 h after transfection, cell lysates were prepared and immunoprecipitated. (C) HEK293-FcγRII cells were cotransfected with plasmids encoding GFP-DrrA_61–647 and 3x-FLAG constructs. 24 h after transfection, cell lysates were prepared and immunoprecipitated. (D) HEK293-FcγRII cells were transfected with a plasmid encoding 3x-FLAG-Sec15. 24 h after transfection, cells were infected with wild-type Legionella or ΔdrrA mutant strain for 1 h at MOI 50. After infection, cell lysates were prepared and immunoprecipitated.