Table 1. Quantification of mitotic cells after colchicine treatment.

		WT	mad2Δ	aldG4422
pH3-positive cells per intestinal region	Sucrose	0.29 ± 0.85	3.11 ± 3.90	5.73 ± 4.82
	Colchicine	34.81 ± 30.46****	12.62 ± 12.60	34.27 ± 19.74
Mitotic index fold change (sucrose vs. colchicine)		×120	×4	×6

Quantification of pH3-positive cells present in the first two fields of view of the posterior midgut after the pyloric ring (40× objective) of WT and homozygous mutants for mad2 or mps1. n (intestines) = 24 for WT on sucrose; n (intestines) = 47 for WT on sucrose + colchicine; n (intestines) = 22 for mad2 mutants on sucrose; n (intestines) = 29 for mad2 mutants on sucrose + colchicine; n (intestines) = 11 for mps1 mutants on sucrose; n (intestines) = 11 for mps1 mutants on sucrose + colchicine. ****, P < 0.0001; Mann–Whitney U test.