Figure 4.

In vitro–transcribed naturally intronless mRNAs without a polyA tail are accumulated in NSs. (A) Top: Schematic of reporter constructs. Sequences from the vector and the genes of interest are indicated as cyan line and gray bar, respectively. The positions of the cap, polyA tail (An), and FISH probe are indicated. Confocal microscopic images show the distribution of microinjected cG, HSPA1A, GPR119, and JUND mRNAs as well as NSs. FISH with the 5′ vector probe and SRSF2 IF were performed 30 min after microinjection. Bar, 20 µm. The red and green lines in the graphs demonstrate the mRNA and SRSF2 IF signal intensity, respectively. The colocalization indexes are shown on the right. Data represent the mean ± SD from three independent experiments; n = 10. Statistical analysis was performed by using the unpaired Student’s t test. ***, P < 0.01. CDS, coding sequence. (B) Same as in A, except that in vitro–transcibed but not polyadenylated intronless mRNAs were used for microinjection.