Figure 7.

SOX13 regulates NKT17 cell development. (A) Representative profiles showing the reduction of thymic iNKT cells and LN RORγt+ NKT17 cells in Sox13^−/− (KO, ∼3–4 wk old, 129/J background) and aged-matched 129/J WT mice. One of two experiments. (B) Summary of the frequencies of total iNKT cells and NKT cell subsets defined by TFs in the thymus and LN of KO relative to WT mice. Each dot represents an individual mouse. Total tissue cellularity of KO and WT mice was indistinguishable. Data are combined from two independent experiments. (C) Representative profiles of effector cytokine (IL-4, IL-17A, and IFNγ) production by LN iNKT cells from WT and KO mice as measured by intracellular staining. One of two experiments with a minimum of n = 3/genotype is shown. (D) Thymic iNKT cells were stimulated with PMA and Ionomycin for simultaneous analysis of TFs and cytokine production. NKT17 cells were gated as live, B220/CD19⁻, TCRβ⁺, mCD1d-PBS57⁺, and RORγt⁺T-bet⁻. Data are summarized from two independent assays. Error bars denote SD.