Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Nov 5;215(11):2833–2849. doi: 10.1084/jem.20180439

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2018 Wang et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 7. — The effect of Methylstat and GDC-0941 combination in vivo. (A) NOD-SCID mice bearing MB436 xenografts were treated with vehicle (n = 13) and Methylstat (30 mg/kg; n = 12) by intraperitoneal injection twice a week, GDC-0941 (100 mg/kg; n = 15) by oral gavage daily, or both drugs (n = 6) for 2 wk. Tumor volumes were measured twice a week. (B) Western blot analysis of representative tumors from each experimental group in A. MW, molecular weight. (C) NOD-SCID mice bearing MB436 (TetOn-shKDM4B) xenografts were fed with diets of doxycycline or treated with GDC-0941 (100 mg/kg) by oral gavage daily or in combination as indicted for 1 mo. Tumor volumes were measured twice a week. Vehicle (n = 9), doxycycline (n = 9), GDC-0941 (n = 10), combination (n = 10). (D) Western blot analysis of representative tumors from each experimental group in C. (E) NOD-SCID mice bearing PTEN-deficient PDX#41 were treated with vehicle (n = 6) and ML324 (60 mg/kg; n = 7) by intraperitoneal injection daily, GDC-0941 (100 mg/kg; n = 7) by oral gavage daily, or both drugs (n = 6) for 2 wk. NOD-SCID mice bearing PTEN wild-type PDX#EL12-58 were treated with vehicle (n = 6), ML324 (60 mg/kg; n = 6), GDC-0941 (100 mg/kg; n = 6), or both drugs (n = 6) for 2 wk. Tumor volumes were measured twice a week. (F) Western blot analysis of representative tumors from each experimental group in E. See also Fig. S5. Data are presented as mean ± SEM. P values were determined by two-tailed unpaired Student’s t test; ns P > 0.05, * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001, **** P ≤ 0.0001.