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. 2018 Jun 22;32(12):6911–6922. doi: 10.1096/fj.201800597R

Figure 3.

Figure 3

HG exosomes increase levels of miR-28, -31a, and -130a in DRG neurons. A) Heat maps of miRs acquired from Taqman miR array analysis show the top 20 highly expressed miRs ranked by Ct values in HG exosomes. Levels of these miRs were compared between HG exosomes and control exosomes (left column), and between HG-Schwann cells and control-Schwann cells (right column). B, C) Asterisks indicate 2-fold change of miRs. qRT-PCR analysis showed that transfection of Schwann cells with siRNAs against miR-28, -31a, and -130a abolished HG-elevated these 3 miRs in HG exosomes (B) and in Schwann cells (C). D) Treatment of distal axons with HG exomes derived from Schwann cells transfected with siRNA against these 3 miRs did not increase levels of these 3 miRs within the axons. E, F) Application of HG exosomes into the distal axons elevated levels of miR-28, -31a, and -130a in distal axons (E), but not in cell bodies of DRG neurons (F). F–I) qRT-PCR (F) and FISH (G–I) analysis showed that application of HG exosomes into cell bodies and distal axons increased miR-28, -31a, and -130a in entire DRG neurons (n = 6 chambers/3 individual experiments /group). Scale bars, 10 μm. Asterisks denote fold change ≥2 (A) or P < 0.05 (B–F).