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. 2018 Jun 22;32(12):6911–6922. doi: 10.1096/fj.201800597R

Figure 5.

Figure 5

HG exosomes reduce protein levels of DNMT3A, NUMB, SNAP25, and GAP43 in distal axons of DRG neurons. Inhibition and elevation of miR-28, -31a, and -130a in distal axons were inversely related to these protein levels. A, B) Representative Western blot images of DNMT3A, NUMB, SNAP25, and GAP43 (A) and quantitative data of these proteins (B) in distal axons treated with HG exosomes or left untreated under regular glucose condition. C–H) Transfection of axons with siRNA against miR-28 (C), -31a (E), and -130a (G) or with mimics of miR-28 (C), -31a (E), and -130a (G) increased or decreased the protein levels of DNMT3A (D), NUMB (F), and GAP43 and SNAP25 (H) (n = 6 chambers/3 individual experiments/group). *P < 0.05.