Figure 2.

A, D) Crat in AgRP neurons is needed to regulate nutrient partitioning and energy expenditure in response to CR. BAT temperature (A) and locomotor activity (D) under ad libitum fed and CR conditions shown as a heat map. Each voxel represents average BAT temperature or cumulative locomotor activity of 15-min intervals, each column represents a day, the black bar on the left-hand side indicates the dark phase, the green bar on top of the heat map indicates ad libitum access to food, and the red bar indicates restricted access to 1.8 g/24 h starting at 11 zeitgeber time (ZT), indicated by the horizontal dotted line. B, C) Average BAT temperature during the light and dark phases of ad libitum fed animals (B) and regression analysis of average BAT temperature during the dark phase of 9 consecutive days CR (C). E, F) Total locomotor activity during the light and dark phases of ad libitum fed animals (E) and regression analysis of total dark phase locomotor activity under CR conditions (F). G–J) Average 24-h profile of energy expenditure (G) and respiratory quotient (RQ) (I) of CR d 7–9, and light and dark phase average of energy expenditure (H) and RQ (J) of this period. All data are expressed as means ± sem; n = 5–7. P < 0.05 (ANOVA with Sidak post hoc analysis, 2-way repeated measures, where appropriate).