Figure 5.

Trpm2^−/− neutrophils are defective in NET-mediated destruction of bacteria in vitro. A) WT and Trpm2^−/− neutrophils were stimulated with 10 mM H₂O₂ to induce NET formation and subsequently infected with KPn (multiplicity of infection = 10) in the presence of cytochalasin D with or without DNase I for 90 min. Data presented in the bar graph is the average ± se of 4 independent experiments. **P < 0.01. B) Representative fluorescent images of various treatments described in A are shown (original magnification, ×100).