Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Jun 19;32(12):6760–6770. doi: 10.1096/fj.201800244RR

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution 4.0 International (CC BY 4.0) (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

DUSP14 methylation induces its binding to TRAF2. A) The interaction between DUSP14 and TRAF2 was inhibited by PRMT5 shRNA knockdown. Myc-TRAF2, Flag-DUSP14, and PRMT5 shRNA #2 were cotransfected into HEK293T cells. The cell lysates were immunoprecipitated with anti-Flag antibody and then immunoblotted with anti-Myc or anti-Flag antibody. B) DUSP14 methylation–defective mutant did not bind to TRAF2. Flag-TRAF2 plus either Flag-DUSP14 wild-type or mutant (3R→K) was transfected into HEK293T cells. The cell lysates were immunoprecipitated with anti-DUSP14 antibody and then immunoblotted with anti-Flag or anti-DUSP14 antibody. C) DUSP14 methylation enhanced its interaction with the E3 ligase TRAF2. Flag-TRAF2 plus either Flag-DUSP14 wild-type or mutant (Q29N/T31S) were cotransfected into HEK293T cells. The cell lysates were immunoprecipitated with anti-DUSP14 antibody and then immunoblotted with anti-TRAF2 or anti-DUSP14 antibody. Arrowhead indicates the DUSP14 protein (upper band of the doublet). Data shown are representative of 3 independent experiments.