Figure 3.

CS-exposed rSPLUNC1 fails to internalize ENaC. HEK293T cells were transiently transfected with ENaC subunits (i–iii) as indicated and studied 24 h later. Representative immunoblots and densitometry of membrane and whole-cell protein levels, respectively: α-ENaC (A), β-ENaC (B), and γ-ENaC (C). Whole-cell lysate results are presented as samples normalized to Ringer’s control. GAPDH (glyceraldehyde 3-phosphate dehydrogenase) was blotted as a loading control for the lysate. Each symbol represents 1 culture (n = 6 from 6 different experiments). *P < 0.05 (ANOVA).