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. 2018 Jun 11;32(12):6559–6574. doi: 10.1096/fj.201800345R

Figure 5.

Figure 5

SPLUNC1 cysteine mutants are required for ASL volume homeostasis. A, B) HBECs were loaded with 20 µl Ringer’s solution that contained 1 mg/ml of 10-kDa rhodamine-dextran and 40 µM rSPLUNC1, rSPLUNC1C180A, and rSPLUNC1C224A as indicated (A), and ASL height was measured by XZ-confocal microscopy (B; all: n = 9 from 3 donors). C–H) rSPLUNC1C180A (C, D), rSPLUNC1C224A (E, F), and rSPLUNC1C180/C224A (G, H) were exposed to CS and cultures were subsequently loaded with 40 µM of each protein. Representative XZ-confocal micrographs of ASL height (red) 6 h after addition of rSPLUNC1 or rSPLUNC1 mutants (C, E, G), and the mean ASL heights are shown (D, F, H; n = 9 from 3 donors). Scale bars, 5 µm. *P < 0.01, **P < 0.001 (ANOVA).