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. 2018 Jun 19;32(12):6883–6897. doi: 10.1096/fj.201800413RR

Figure 2.

Figure 2

JNK and Akt phosphorylation are altered concomitant with increased cell death in the retina of mice fed a Western diet. C57BL/6 mice were fed either a Western diet (WD) or control diet (CD) diet. A–C) JNK phosphorylation on Thr183/Tyr185 (A) and phosphorylation of Akt on Ser473 (B) and Thr308 (C) were assessed in retinal lysates obtained after 14 wk of diet by Western blot analysis. Representative blots are shown. Protein molecular mass (kDa) is indicated at the right of the blots. D) Apoptotic cell death was assessed by ELISA in retinal lysates after 18 wk of diet for the presence of nucleosomal fragments in the cytoplasm. Results are means ± sem (n = 4–7). *P < 0.05, ***P < 0.01 vs. control diet.