TABLE 1.
Antibodies used for Western blot analysis
| Antibody | Catalog no. | Lot no. | Molecular mass (kDa) | Species | Specificity |
|---|---|---|---|---|---|
| Cell Signaling Technology (Danvers, MA, USA) | |||||
| AKT-p (S473) | 4060 | 19 | 60 | Rabbit | H, M, R |
| AkT-p (T308) | 9275 | 20 | 60 | Rabbit | H, M, R |
| AKT | 9272 | 27 | 60 | Rabbit | H, M, R |
| JNK-p (T183/Y185) | 9251 | 24 | 46, 54 | Rabbit | H, M, R |
| JNK | 9252 | 15 | 46, 54 | Rabbit | H, M, R |
| c-Jun-p (S63) | 9261 | 14 | 48 | Rabbit | H, M, R |
| c-Jun | 9165 | 9 | 43, 48 | Rabbit | H, M, R |
| p38 MAPK-p (Thr180/Tyr182) | 4511 | 13 | 43 | Rabbit | H, M, R |
| p38 MAPK | 8690 | 6 | 40 | Rabbit | H, M, R |
| Cleaved caspase 3 Asp175 | 9664 | 21 | 17, 19 | Rabbit | H, M, R |
| TXNIP | 14715 | 1 | 55 | Rabbit | H, M, R |
| Actin | 3700 | 14 | 46 | Mouse | H, M, R |
| Santa Cruz Biotechnology (Dallas, TX, USA) | |||||
| GAPDH | sc-32233 | K0315 | 37 | Mouse | H, M, R |
| α-Tublin | sc-32293 | C0112 | 55 | Mouse | H, M, R |
| Protein Tech (Manchester, United Kingdom) | |||||
| REDD1 | 10638-1-AP | 51272 | 35 | Rabbit | H, M, R |
| Origene Technologies (Rockville, MD, USA) | |||||
| DDK (FLAG tag) | TA5011-100 | A043 | - | Mouse | M |
| Bethyl Laboratories (Montgomery, TX, USA) | |||||
| Secondary antibody | A120-101P | 40 | - | Goat | R |
| Secondary antibody | A90-116P | 38 | - | Goat | M |
Primary and secondary antibodies used for Western blot analysis are listed. Antibody specificity to human (H), mouse (M), and rat (R) is also indicated.