The spleen (SPL) is the primary reservoir of infiltrating monocytes in the TEVGs. A, B) Representative dot plots (A) and absolute numbers (B) of Ly-6C+hi monocytes in TEVGs from WT (SPL+/+) and splenectomized (SPL−/−) animals. C, D) Population of Ly-6C+hi monocytes (C) and their absolute numbers (D) in spleens from CD45.1 CX3CR1gfp/gfp mice before transplantation (SPL control), and 0 or 3 d postimplantation (DPI). E, F) Dot plots (E) and total numbers (F) of Ly6C+hi/CXC3CR1+ monocytes in TEVGs from CD45.2 WT, CD45.1 CX3CR1gfp/gfp, and CD45.2 WT mice receiving a CD45.1 CX3CR1gfp/gfp spleen. Means ± sem; n = 6/group. **P < 0.01, ***P < 0.001.