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. 2018 Jun 20;32(12):6358–6370. doi: 10.1096/fj.201800691R

Figure 5.

Figure 5

PAD4 activity/NETosis increase with age, and PAD4/NETs are implicated in age-related organ fibrosis. A) More circulating neutrophils from old (age 14–17 mo) wild-type (WT) mice were H3Cit positive compared with those of young (age 1.5–3.5 mo) mice. B) Compared with young WT mice, a higher percentage of neutrophils from old WT mice produced NETs spontaneously, as well as in the presence of stimulants, after incubation ex vivo. C) Sirius red staining revealed more interstitial collagen deposition (red, arrowheads) in the heart of old WT mice than in age-matched PAD4−/− mice. Scale bar, 100 μm. Iono, ionomycin; PMA, phorbol 12-myristate 13-acetate; US, unstimulated. From Martinod et al. (113).