Key resources table.
Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
---|---|---|---|---|
Strain, strain background (Escherichia coli) |
BL21 DE3 | New England Biolabs | C2527 | |
Strain, strain background (E. coli) |
K12 strain (MG1655) | doi: 10.1128/JB.188.3.928–933.2006 | Dr. Matthew Mulvey, Univ. of Utah |
|
Strain, strain background (Aeromonas veronii) |
HM21S; Aer; Aeromonas Hm21; Hm21 |
doi:10.1128/AEM.01621–10 | Parent strain, SmR | |
Strain, strain background (A. veronii) |
HE-1095; Aer DT2 | doi:10.1128/AEM.01621–10 | Hm21S::interrupted exeM mTn5 KmR SmR |
|
Strain, strain background (A. veronii) |
HEC-1344; Aer DT2C | doi:10.1128/AEM.01621–10 | HE-1095::Tn7 containing TpR exeMN + promoter region |
|
Strain, strain background (A. veronii) |
ASRC7; Aer DaimA | this study | Hm21S aimA::cmR | |
Strain, strain background (A. veronii) |
ASRD5; Aer DaimB | this study | Hm21S DaimB | |
Strain, strain background (A. veronii) |
ASRD4; Aer DADB |
this study | Hm21S aimA::cmR;
DaimB |
|
Strain, strain background (A. veronii) |
ZOR0001; ZF Aer |
doi:10.1038/ismej.2015.140 | Zebrafish Aeromonas
isolate |
|
Strain, strain background (A. veronii) |
ZOR0001; ZF Aer DaimA |
this study | ZOR0001 aimA::cmR | |
Genetic reagent (Danio rerio) |
AB x Tu strain; wild type zebrafish |
UO Zebrafish facility | ||
Genetic reagent (Danio rerio) |
myd88-/- | PMID: 28973938 | ||
Genetic reagent (Danio rerio) |
Tg(BACmpx:GFP) i114; mpx:GFP |
doi:10.1182/blood -2006-05-024075 |
||
Recombinant DNA reagent |
pET21B | Genscript | ||
Chemical compound, drug |
LPS, E. Coli
O111:B4 |
Sigma | L2630 | |
Peptide, recombinant protein |
Lipocalin 2, LCN2, Siderocalin |
Biolegend | 588002 | |
Peptide, recombinant protein |
AimA | this study | NCBI hypothetical protein WP_021230730.1 |
see Materials and Methods |
Peptide, recombinant protein |
AimB | this study | NCBI hypothetical protein WP_021230165.1 |
see Materials and Methods |
Peptide, recombinant protein |
mLCN2 | this study | NCBI gene NM_008491.1 | see Materials and Methods |