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. Author manuscript; available in PMC: 2019 Nov 15.
Published in final edited form as: J Immunol. 2018 Oct 5;201(10):2910–2922. doi: 10.4049/jimmunol.1700638

Figure 4. Helminth-induced suppression of inflammatory cytokine generation in BMT mice requires host cell IL4 production.

Figure 4.

Concentrations of IFNγ (A) and TNFα (B) in supernatants of splenic donor T cell cultures isolated from uninfected or Hpb-infected IL4−/− or WT BALB/c BMT mice were assessed by ELISA. WT donor T cells were FACS-sorted as described in Materials and Methods and seeded in triplicate at 105 cells per well. Wells were coated in anti-CD3 and cells were cultured with additional soluble anti-CD28. Serum concentrations of IFNγ (C) and TNFα (D) in uninfected and Hpb-infected animals six days after BMT were assessed by ELISA. Each symbol (dot) represents an independent experiment (N) and is calculated as the average of ≥3 wells from an individual mouse; bars represent the mean from multiple samples; p values <0.05 between uninfected and Hpb-infected as indicated for each panel; differences between groups determined by unpaired Welch’s t-test.