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. Author manuscript; available in PMC: 2019 Nov 5.
Published in final edited form as: Dev Cell. 2018 Oct 11;47(3):281–293.e4. doi: 10.1016/j.devcel.2018.09.015

Figure 4. Loss of hermes does not influence caspases during salivary gland degradation.

Figure 4.

(A) Samples from hrm mutant animals (w; hrmΔ1/Df(2R)BSC696; fkh-GAL4/+), n=20 (left), animals with salivary gland-specific expression of p35 (w; hrmΔ1/+; fkh-GAL4/UAS-p35), n=19 (middle), and hrm mutants with salivary gland-specific expression of p35 (w; hrmΔ1/Df(2R)BSC696; fkh-GAL4/UAS-p35), n=16 (right), analyzed by histology for the presence of salivary gland material (red dotted circle) 24h after puparium formation. Arrows indicate persisting salivary gland luminal structures. See also Figure S4. (B) Quantification of data from (A). Statistical significance was determined using a Chi-square test comparing the percentages of gland fragments. (C) Salivary glands dissected from control (w; hrmΔ1/+; +), n=4 (left), animals and hrm mutant animals (w; hrmΔ1/Df(2R)BSC696; +), n=8 (right), 13.5h after puparium formation and stained with cleaved Caspase-3 antibody (green) and Hoechst (blue). Scale bars represent 20μm. (D) Quantification of data from (C). Data are represented as mean ± SEM. Statistical significance was determined using a Student’s t test.