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. Author manuscript; available in PMC: 2019 Nov 5.
Published in final edited form as: Dev Cell. 2018 Oct 11;47(3):281–293.e4. doi: 10.1016/j.devcel.2018.09.015

Figure 7. hermes alters lipid metabolism and mTor signaling.

Figure 7.

(A) Salivary glands dissected from control animals (w; hrmΔ1/+; +), n=13, (left) and hrm mutant animals (w; hrmΔ1/Df(2R)BSC696; +), n=13, (right) 13.5h after puparium formation and stained with Nile Red (red) and Hoechst (blue). Scale bars represent 20μm. (B) Quantification of data from (A). Data are represented as mean ± SEM. Statistical significance was determined using a Student’s t test. (C) Western blot analyses of phospho-S6K and actin 13.5 h after puparium formation in salivary gland extracts from control (w; hrmΔ1/+; +), n=8, and hrm mutant animals (w; hrmΔ1/Df(2R)BSC696; +), n=8. (D) Quantification of data from (C). All samples are normalized to actin and plotted relative to control sample. Data are represented as mean ± SEM. (E) Samples from control hrm mutant animals (w; hrmΔ1/hrmΔ1, UAS-GFP; sg-GAL4/+), n=11 (left) and hrm mutants specifically expressing torTED in the salivary glands (w; hrmΔ1, UAStorTED/hrmΔ1, UAS-GFP; sg-GAL4/+), n=20 (right), analyzed by histology for the presence of salivary gland material (red dotted circle) 24h after puparium formation. (F) Quantification of data from (E).