Fig. 2.

From harvest to finished product. (1) A small blood sample (15 cc) is acquired through venipuncture; (2) the collected sample is centrifuged (5 min; 5000 rpm); (3) after the centrifugation is complete, the sample is ready to be separated; (4) using the double-syringe system, the plasma layer is separated from the lower layer containing leukocytes and erythrocytes; (5) the inner syringe, now containing the ACP, is separated from the outside syringe, which contains the discarded leukocytes and erythrocytes; (6) once a needle is attached, the ACP is ready to be injected