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. 2018 Oct 31;9:2532. doi: 10.3389/fimmu.2018.02532

Figure 1.

Figure 1

CLP induces transient reductions in the number of B cells and CD4 T cells. (A) Experimental design—blood and secondary lymphoid organs (SLO) including spleens, inguinal lymph nodes (iLN), and mesenteric lymph nodes (mLN) were harvested 2 and 30 days after sham or CLP surgery. The number of (B,C) CD19+ B220+ B cells or (D,E) CD3+ CD4+ T cells in blood (per ml), spleen, iLN, and mLN was determined by flow cytometry. n = 5–7 mice/group. **p < 0.01, ****p < 0.001. Numbers above bars indicate the average fold change in number compared to sham mice. Data are representative of at least 3 independent experiments.