FIGURE 2.

Eicosanoid biosynthetic genes are upregulated upon infection with S. Typhimurium or Y. enterocolitica. Targeted RT-PCR of eicosanoid biosynthetic genes was performed on THP-1-derived macrophages either uninfected/infected with S. Typhimurium (A) or Y. enterocolitica (B) for 2 h at an MOI of 50:1. For comparison, isogenic strains of S. Typhimurium lacking the T3SS (ΔssaV) and Y. enterocolitica lacking the pYV virulence plasmid (8081c) were used to infect THP-1 cells. Two-step RT-PCR was performed on a Stratagene MXP3005 using SYBRGreen reagents. Fold change was calculated using the ΔΔCt method. Two-way ANOVA along with Tukey’s post hoc tests were used to calculate significance (N = 3). p-values were indicated as follows: ^∗p ≤ 0.05; ^∗∗p ≤ 0.01; ^∗∗∗p ≤ 0.001; ^∗∗∗∗p ≤ 0.0001.