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. 2018 Oct 31;9:2447. doi: 10.3389/fmicb.2018.02447

FIGURE 3.

FIGURE 3

Levels of eicosanoids secreted into culture medium by macrophages infected with S. Typhimurium or Y. enterocolitica. Triple quadrupole mass spectrometry-based targeted metabolomics study was performed on cell culture supernatants of THP-1 macrophages infected with S. Typhimurium or Y. enterocolitica for 2 h at an MOI of 50:1. The media extracts were analyzed for AA, PGF2α, TxB2, and PGE2/PGD2, and relative abundance was calculated using internal standards (N = 3). Significance was calculated by using Student’s t-test. p-values were indicated as follows: ∗p ≤ 0.05; ∗∗p ≤ 0.01; ∗∗∗p ≤ 0.001; ∗∗∗∗p ≤ 0.0001.