Table 3.

Summary of residues investigated in this study and their relation to antigenicity and avidity

^aInverse Simpson index, 1.00 equivalent to 100% conserved, numbers above 1.00 indicate increasing levels of diversity; ^bPublished mAb escape mutant, citations in Table S1. ^cIdentified by integrated modelling of HI titres and gene sequence data (Table 2). ^dSignificant impact on HI titre as shown in Figs. 1 and/or 3. Estimated impact of antigenicity and avidity change on HI titre if reciprocal mutations were analysed (Fig. 2). Substitutions associated with absent or almost absent (frequency < 1% among sequenced viruses) are shown in italicised font. Substitutions identified by mAb escape study or modelling but shown not to impact HI titres are indicated by strikethrough. * Potential to add glycosylation site. Shaded cells indicate not tested