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. 2018 Nov 7;7:175. doi: 10.1038/s41426-018-0177-z

Fig. 3. KFDV can infect and replicate in human moDCs, leading to increased IL-6 and TNF-α production.

Fig. 3

a PBMCs were obtained from buffy coats from 5 healthy and naïve donors (a–e). Monocytes were purified from PBMCs by positive magnetic separation. MoDCs were obtained and infected with KFDV as described in the Materials and Methods, and viral growth was quantified in cell culture supernatants at 0, 24, 48, and 72 h p.i. using the plaque assay. b–d Supernatants from uninfected (control), KFDV-infected and mock-infected (UV-inactivated virus) moDCs were collected at 24 (b), 48 (c), and 72 (d) h p.i., and levels of IL-6 were determined by ELISA. e–g Supernatants from KFDV-infected and mock-infected (UV-inactivated virus) moDCs were collected at 24 (b), 48 (c), and 72 (d) h.p.i., and levels of TNF-α were determined by ELISA. Data represent mean concentrations ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001